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RBM22 Polyklonaler Antikörper

RBM22 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22103-1-PBS

Synonyme

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  • Western Blot (WB) analysis of Raji cells using RBM22 Polyclonal antibody (22103-1-AP)

    WB analysis of Raji using 22103-1-AP (same clone as 22103-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RBM22 Polyclonal antibody (22103-1-AP)

    WB analysis using 22103-1-AP (same clone as 22103-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using RBM22 Polyclonal antibody (22103-1-AP)

    WB analysis of NIH/3T3 using 22103-1-AP (same clone as 22103-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RBM22 Polyclonal antibody (22103-1-AP)

    IF Staining of U2OS using 22103-1-AP (same clone as 22103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM22 antibody (22103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

22103-1-PBS bindet in WB, IF/ICC, Indirect ELISA RBM22 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RBM22 fusion protein Ag17315
Vollständiger Name RNA binding motif protein 22
Berechnetes Molekulargewicht 420 aa, 47 kDa
Beobachtetes Molekulargewicht 47-50 kDa
GenBank-ZugangsnummerBC003402
Gene symbol RBM22
Gene ID (NCBI) 55696
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM22 is mainly involved in pre-mRNA splicing, playing the essential role of maintaining the conformation of the catalytic core of the spliceosome and acting as a bridge between the catalytic core and other essential protein components of the spliceosome. RBM22 is also involved in gene regulation, and is able to bind DNA, acting as a bona fide transcription factor on a large number of target genes. Due to its wide scope in the regulation of gene expression, RBM22 has been associated with several pathologies and, notably, with the aggressiveness of cancer cells and with the phenotype of a myelodysplastic syndrome. (PMID: 35158909)