RBM24 Polyklonaler Antikörper

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• KD/KO Validated

RBM24 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 18178-1-AP

Synonyme

RBM24, RNA binding motif protein 24, RNA binding protein 24, RNPC6

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Galerie der Validierungsdaten

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  WB analysis using 18178-1-AP

  mouse heart tissue lysates were subjected to SDS PAGE followed by western blot with 18178-1-AP (RBM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18178-1-AP (RBM24 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 18178-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 18178-1-AP (RBM24 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 18178-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 18178-1-AP (RBM24 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse skeletal muscle using 18178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using RBM24 antibody (18178-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse skeletal muscle using 18178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using RBM24 antibody (18178-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• RIP: See 1 publications below

Produktinformation

18178-1-AP bindet in WB, RIP, IHC, IF, ELISA RBM24 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM24 fusion protein Ag12871
• Vollständiger Name: RNA binding motif protein 24
• Berechnetes Molekulargewicht: 191 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20-27 kDa
• GenBank-Zugangsnummer: BC104808
• Gene symbol: RBM24
• Gene ID (NCBI): 221662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA-binding proteins have a pivotal role in post-transcriptionally regulating gene expression. During early development they exhibit temporally and spatially regulated expression pattern, and affect the proteome of a single cell by controlling alternative splicing, transport, localization, and stability of their target RNAs [PMID:22510371]. Their expression are tissue-specific, and their crucial influence on the protein set-up especially in muscle cells has become recognized recently [PMID:21982546]. RBM24 is one RNA-binding protein and plays a fundamental role in regulating cardiac gene expression, sarcomeric assembly, and cardiac contractility and may thus represent a novel pathway to cardiomyopathy [PMID:20338237].

Publikationen

Species	Application	Title
mouse	IF	Elife; Dual expression of Atoh1 and Ikzf2 promotes transformation of adult cochlear supporting cells into outer hair cells.; Authors - Suhong Sun
mouse	IF	Development; Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes.; Authors - Guangqin Wang
mouse	IF	Sci Rep; Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration.; Authors - Raphaëlle Grifone ; KD Validated
mouse	IF	Dev Dyn; Expression patterns of Rbm24 in lens, nasal epithelium and inner ear during mouse embryonic development.; Authors - Raphaëlle Grifone
chicken, mouse	IF	Mech Dev; The RNA-binding protein Rbm24 is transiently expressed in myoblasts and is required for myogenic differentiation during vertebrate development.; Authors - Raphaëlle Grifone
human	IHC,RIP	J Mol Med (Berl); Mechanism of METTL14-mediated m^6A modification in non-small cell lung cancer cell resistance to cisplatin; Authors - Shulei Gong