RBM24 Polyklonaler Antikörper

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• KD/KO Validated

RBM24 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF-P, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 18178-1-AP

Synonyme

RNA binding motif protein 24, RNA binding protein 24, RNA-binding motif protein 24, RNA-binding protein 24, RNA-binding region-containing protein 6

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  WB analysis using 18178-1-AP

  mouse heart tissue lysates were subjected to SDS PAGE followed by western blot with 18178-1-AP (RBM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 18178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18178-1-AP (RBM24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 18178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18178-1-AP (RBM24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 18178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using RBM24 antibody (18178-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse skeletal muscle using 18178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using RBM24 antibody (18178-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• RIP: See 1 publications below

Produktinformation

18178-1-AP bindet in WB, IHC, IF-P, RIP, ELISA RBM24 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM24 fusion protein Ag12871
• Vollständiger Name: RNA binding motif protein 24
• Berechnetes Molekulargewicht: 191 aa, 20 kDa
• Beobachtetes Molekulargewicht: 18-25 kDa
• GenBank-Zugangsnummer: BC104808
• Gene symbol: RBM24
• Gene ID (NCBI): 221662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA-binding proteins have a pivotal role in post-transcriptionally regulating gene expression. During early development, they exhibit temporally and spatially regulated expression patterns and affect the proteome of a single cell by controlling alternative splicing, transport, localization, and stability of their target RNAs (PMID: 22510371). Their expression is tissue-specific, and their crucial influence on the protein setup, especially in muscle cells, has become recognized recently (PMID: 21982546). RBM24 is one RNA-binding protein that is fundamental in regulating cardiac gene expression, sarcomeric assembly, and cardiac contractility. It may thus represent a novel pathway to cardiomyopathy (PMID: 20338237).

Publikationen

Species	Application	Title
mouse	WB,IHC	Int J Biol Macromol; Rbm24 modulates neuronal RNA splicing to restrict cognitive dysfunction; Authors - Dongbo Yao
mouse	IF	Elife; Dual expression of Atoh1 and Ikzf2 promotes transformation of adult cochlear supporting cells into outer hair cells.; Authors - Suhong Sun
mouse	IF	Development; Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes.; Authors - Guangqin Wang
mouse	IF	Sci Rep; Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration.; Authors - Raphaëlle Grifone ; KD Validated
mouse	IF	Dev Dyn; Expression patterns of Rbm24 in lens, nasal epithelium and inner ear during mouse embryonic development.; Authors - Raphaëlle Grifone
mouse,chicken	IF	Mech Dev; The RNA-binding protein Rbm24 is transiently expressed in myoblasts and is required for myogenic differentiation during vertebrate development.; Authors - Raphaëlle Grifone