RBM25 Rekombinanter Antikörper

RBM25 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242526B7

Kat-Nr. : 84968-4-PBS

Synonyme

242526B7, Arg/Glu/Asp-rich protein of 120 kDa, NET52, Protein S164, RBM 25

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  WB analysis using 84968-4-RR (same clone as 84968-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84968-4-RR (RBM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84968-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84968-4-RR (same clone as 84968-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84968-4-RR (RBM25 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84968-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84968-4-RR (same clone as 84968-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84968-4-RR (RBM25 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84968-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84968-4-RR (same clone as 84968-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM25 antibody (84968-4-RR, Clone: 242526B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84968-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84968-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84968-4-RR against Human RBM25 were performed. The affinity constant is 2.38 nM.

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Geprüfte Anwendungen

Produktinformation

84968-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RBM25 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RBM25 fusion protein Ag18244
• Vollständiger Name: RNA binding motif protein 25
• Berechnetes Molekulargewicht: 843 aa, 100 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC113389
• Gene symbol: RBM25
• Gene ID (NCBI): 58517
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.