RBM39 Monoklonaler Antikörper

RBM39 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D2C8

Kat-Nr. : 67420-1-PBS

Synonyme

CAPER, CAPERalpha, CC1.3, DKFZp781C0423, FLJ44170, fSAP59, HCC1, RBM39, RNA binding motif protein 39, RNA binding protein 39, RNPC2, Splicing factor HCC1

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  WB analysis using 67420-1-Ig (same clone as 67420-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67420-1-Ig (RBM39 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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  WB analysis using 67420-1-Ig (same clone as 67420-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67420-1-Ig (RBM39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67420-1-Ig (same clone as 67420-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67420-1-Ig (RBM39 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67420-1-Ig (same clone as 67420-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67420-1-Ig (RBM39 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67420-1-Ig (same clone as 67420-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67420-1-Ig (RBM39 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67420-1-Ig (same clone as 67420-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM39 antibody (67420-1-Ig, Clone: 2D2C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67420-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67420-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RBM39 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBM39 fusion protein Ag15766
• Vollständiger Name: RNA binding motif protein 39
• Berechnetes Molekulargewicht: 530 aa, 59 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa
• GenBank-Zugangsnummer: BC141835
• Gene symbol: RBM39
• Gene ID (NCBI): 9584
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM39, also named as HCC1 or RNPC2, is a 530 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the splicing factor SR family. RBM39 is widely expressed in various tissues and with highly expression in pancreas, skeletal muscle, lung and brain. RBM39 is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. RBM39 may be involved in pre-mRNA splicing process.