RBM42 Polyklonaler Antikörper

RBM42 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15407-1-AP

Synonyme

RBM 42, RNA-binding motif protein 42, RNA-binding protein 42

---

• 

  WB analysis using 15407-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15407-1-AP (RBM42 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15407-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15407-1-AP (RBM42 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15407-1-AP

  IP result of anti-RBM42 (IP:15407-1-AP, 4ug; Detection:15407-1-AP 1:800) with HepG2 cells lysate 1800 ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15407-1-AP bindet in WB, IP, ELISA RBM42 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM42 fusion protein Ag7667
• Vollständiger Name: RNA binding motif protein 42
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC004204
• Gene symbol: RBM42
• Gene ID (NCBI): 79171
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM42, also known as RNA-binding motif protein 42, is a member of the RNA-binding protein family that plays critical roles in post-transcriptional regulation of gene expression. Emerging studies highlight RBM42's involvement in pre-mRNA splicing through its association with the spliceosome complex, where it contributes to the precise removal of introns and assembly of mature mRNA. Beyond splicing, RBM42 has been implicated in cellular stress responses, cell cycle regulation, and apoptosis. Dysregulation of RBM42 is linked to developmental disorders and cancers; for instance, mutations in RBM42 are associated with neurodevelopmental abnormalities, while its overexpression or loss correlates with tumor progression in certain malignancies.