RBM47 Rekombinanter Antikörper

RBM47 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250436H8

Kat-Nr. : 86009-1-RR

Synonyme

NET18, RNA binding motif protein 47, RNA binding protein 47, RNA-binding motif protein 47, RNA-binding protein 47

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  WB analysis using 86009-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86009-1-RR (RBM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 86009-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86009-1-RR (RBM47 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 86009-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86009-1-RR (RBM47 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 86009-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using RBM47 antibody (86009-1-RR, Clone: 250436H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86009-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86009-1-RR against Human RBM47 were performed. The affinity constant is 1.02 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A549-Zellen, Caco-2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86009-1-RR bindet in WB, IHC, IF/ICC, ELISA RBM47 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RBM47 fusion protein Ag20984
• Vollständiger Name: RNA binding motif protein 47
• Berechnetes Molekulargewicht: 593 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC126261
• Gene symbol: RBM47
• Gene ID (NCBI): 54502
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM47, also known as RNA-binding motif protein 47 or RNA-binding protein 47, is a vertebrate-conserved RBP containing three RNA-recognition motifs (RRMs) that perform multifaceted roles in RNA editing, early embryonic development, and cancer.Recent studies suggest that RBM47 functions as a tumor suppressor. It has been shown to suppress the progression of breast and colon cancers. RBM47 represents a molecular switch of cell fate decisions that functions as a regulator of the p53/p21-signaling axis. (PMID: 34499322, PMID: 31511650)