RBM5 Polyklonaler Antikörper

RBM5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 19930-1-AP

Synonyme

RMB5, Renal carcinoma antigen NY-REN-9, Protein G15, LUCA15, H37

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  WB analysis of PC-3 using 19930-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19930-1-AP (RBM5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 19930-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19930-1-AP (RBM5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19930-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19930-1-AP (RBM5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 19930-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19930-1-AP (RBM5 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 19930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 19930-1-AP (RBM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 19930-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 19930-1-AP (RBM5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Lungengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

19930-1-AP bindet in WB, IHC, ELISA RBM5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM5 fusion protein Ag13908
• Vollständiger Name: RNA binding motif protein 5
• Berechnetes Molekulargewicht: 815 aa, 92 kDa
• Beobachtetes Molekulargewicht: 110-115 kDa
• GenBank-Zugangsnummer: BC002957
• Gene symbol: RBM5
• Gene ID (NCBI): 10181
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM5, also named as RNA-binding protein 5, is a 815 amino acid protein, which contains 2 RRM (RNA recognition motif) domains and Belongs to the RBM5/RBM10 family. RBM5 localizes in the nucleus and is a component of the spliceosome A complex. RBM5 regulates alternative splicing of a number of mRNAs and may modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. RBM5 may both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. The phosphorylation of RBM5 protein would influence the detected molecular weight. A 105-110 KDa band was also reported (PMID: 27957556).

Publikationen

Species	Application	Title
human	WB	J Biol Chem; CHIP suppresses the proliferation and migration of A549 cells by mediating the ubiquitination of eIF2α and upregulation of tumor suppressor RBM5; Authors - Bo Jin
mouse	WB	J Neurotrauma; RNA Binding Motif 5 Gene Deletion Modulates Cell Signaling in a Sex-Dependent Manner but not Hippocampal Cell Death.; Authors - Jeffrey Farooq
mouse	WB	Neuroscience; Identification of Novel Targets of RBM5 in the Healthy and Injured Brain.; Authors - Travis C Jackson
mouse	WB	Cell Death Discov; Neuronal RBM5 modulates cell signaling responses to traumatic and hypoxic-ischemic injury in a sex-dependent manner; Authors - Kara Snyder