RBM6 Polyklonaler Antikörper

RBM6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14360-1-PBS

Synonyme

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  WB analysis using 14360-1-AP (same clone as 14360-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14360-1-AP (RBM6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 14360-1-AP (same clone as 14360-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14360-1-AP (RBM6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14360-1-AP (same clone as 14360-1-PBS)

  IP result of anti-RBM6 (IP:14360-1-AP, 4ug; Detection:14360-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14360-1-AP (same clone as 14360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14360-1-AP (RBM6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14360-1-AP (same clone as 14360-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14360-1-AP (RBM6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14360-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA RBM6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM6 fusion protein Ag5743
• Vollständiger Name: RNA binding motif protein 6
• Berechnetes Molekulargewicht: 129 kDa
• Beobachtetes Molekulargewicht: 129 kDa
• GenBank-Zugangsnummer: BC046643
• Gene symbol: RBM6
• Gene ID (NCBI): 10180
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM6, also named as DEF3, is a 1123 amino acid protein, which may interact with FAM168B. RBM6 is expressed ubiquitously in adults tissues. RBM6 specifically binds poly(G) RNA homopolymers in vitro. RBM6 exists some isoforms with MV 129 kDa, 69 kDa, and 59 kDa. onversely, fetal thymus and adult kidney express very little RBM6. In the hemopoietic system of mice, the expression of RBM6 is downregulated upon differentiation of progenitor cells into granulocytes but persists during macrophage development.