RBM7 Polyklonaler Antikörper

RBM7 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (4)

Kat-Nr. : 21896-1-AP

Synonyme

RNA-binding protein 7, RNA-binding motif protein 7, RNA binding protein 7, RNA binding motif protein 7

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  WB analysis of HepG2 using 21896-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21896-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 21896-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 21896-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21896-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21896-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21896-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM7 antibody (21896-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

21896-1-AP bindet in WB, IHC, IF/ICC, ELISA RBM7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM7 fusion protein Ag11106
• Vollständiger Name: RNA binding motif protein 7
• Berechnetes Molekulargewicht: 266 aa, 31 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC034381
• Gene symbol: RBM7
• Gene ID (NCBI): 10179
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM7 (RNA-binding motif protein 7) is an RNA-binding protein initially described to associate with splicing factors, presumably to target intronic RNA for decay (PMID: 35688134). RBM7 regulates the nuclear degradation of NEAT1 non-coding RNA, resulting in sustained apoptosis in the lung epithelium and fibrosis (PMID: 32708315).

Publikationen

Species	Application	Title
		Nat Commun; Temporal-iCLIP captures co-transcriptional RNA-protein interactions; Authors - Ross A Cordiner
human	WB	Mol Cell; ARS2 instructs early transcription termination-coupled RNA decay by recruiting ZC3H4 to nascent transcripts; Authors - Jérôme O Rouvière
human	WB	Nucleic Acids Res; The NEXT complex regulates H3K27me3 levels to affect cancer progression by degrading G4/U-rich lncRNAs; Authors - Qianqian Yang ; KD Validated
human	WB	Mol Cell; P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress.; Authors - Andrii Bugai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (12-16-2024)

Specific antibody!

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: RKO

RBM7 Antibody Western Blot validation (1:3000 dilution) in RKO (Cat no:21896-1-AP)

FH

Tsimafei (Verified Customer) (11-19-2024)

Great antibody that detect Rbm7 in U2OS cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS

RBM7 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:21896-1-AP)

FH

Moritz (Verified Customer) (08-20-2024)

I used this antibody to detect RBM7 with Western blot in HEK293T WT cell lysate, which worked perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T WT

RBM7 Antibody Western Blot validation (1:3000 dilution) in HEK293T WT (Cat no:21896-1-AP)

FH

Roy (Verified Customer) (06-15-2024)

Works well to detect the NEXT complex subunit RBM7 from HeLa extracts (1/1000 Dilution, overnight Incubation at 4°C).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa