RBM7 Polyklonaler Antikörper

RBM7 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 21896-1-AP

Synonyme

RNA-binding protein 7, RNA-binding motif protein 7, RNA binding protein 7, RNA binding motif protein 7



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, HeLa-Zellen
Erfolgreiche Detektion in IHChumanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

KD/KOSee 1 publications below
WBSee 3 publications below

Produktinformation

21896-1-AP bindet in WB, IHC, IF/ICC, ELISA RBM7 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RBM7 fusion protein Ag11106
Vollständiger Name RNA binding motif protein 7
Berechnetes Molekulargewicht 266 aa, 31 kDa
Beobachtetes Molekulargewicht 30-35 kDa
GenBank-ZugangsnummerBC034381
Gene symbol RBM7
Gene ID (NCBI) 10179
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM7 (RNA-binding motif protein 7) is an RNA-binding protein initially described to associate with splicing factors, presumably to target intronic RNA for decay (PMID: 35688134). RBM7 regulates the nuclear degradation of NEAT1 non-coding RNA, resulting in sustained apoptosis in the lung epithelium and fibrosis (PMID: 32708315).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for RBM7 antibody 21896-1-APProtokoll herunterladen
IHC protocol for RBM7 antibody 21896-1-APProtokoll herunterladenl
IF protocol for RBM7 antibody 21896-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle

Nat Commun

Temporal-iCLIP captures co-transcriptional RNA-protein interactions

Authors - Ross A Cordiner
humanWB

Mol Cell

ARS2 instructs early transcription termination-coupled RNA decay by recruiting ZC3H4 to nascent transcripts

Authors - Jérôme O Rouvière
humanWB

Nucleic Acids Res

The NEXT complex regulates H3K27me3 levels to affect cancer progression by degrading G4/U-rich lncRNAs

Authors - Qianqian Yang
  • KD Validated
humanWB

Mol Cell

P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress.

Authors - Andrii Bugai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Aleksandr (Verified Customer) (12-16-2024)

Specific antibody!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: RKO
RBM7 Antibody Western Blot validation (1:3000 dilution) in RKO (Cat no:21896-1-AP)
FH

Tsimafei (Verified Customer) (11-19-2024)

Great antibody that detect Rbm7 in U2OS cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: U2OS
RBM7 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:21896-1-AP)
FH

Moritz (Verified Customer) (08-20-2024)

I used this antibody to detect RBM7 with Western blot in HEK293T WT cell lysate, which worked perfectly.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293T WT
RBM7 Antibody Western Blot validation (1:3000 dilution) in HEK293T WT (Cat no:21896-1-AP)
FH

Roy (Verified Customer) (06-15-2024)

Works well to detect the NEXT complex subunit RBM7 from HeLa extracts (1/1000 Dilution, overnight Incubation at 4°C).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa