RBM7 Polyklonaler Antikörper

RBM7 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21896-1-PBS

Synonyme

RNA-binding protein 7, RNA-binding motif protein 7, RNA binding protein 7, RNA binding motif protein 7

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  WB analysis of HepG2 using 21896-1-AP (same clone as 21896-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 21896-1-AP (same clone as 21896-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21896-1-AP (same clone as 21896-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21896-1-AP (same clone as 21896-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21896-1-AP (RBM7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 21896-1-AP (same clone as 21896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 21896-1-AP (same clone as 21896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 21896-1-AP (same clone as 21896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 21896-1-AP (same clone as 21896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21896-1-AP (RBM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21896-1-AP (same clone as 21896-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM7 antibody (21896-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21896-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21896-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RBM7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBM7 fusion protein Ag11106
• Vollständiger Name: RNA binding motif protein 7
• Berechnetes Molekulargewicht: 266 aa, 31 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC034381
• Gene symbol: RBM7
• Gene ID (NCBI): 10179
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM7 (RNA-binding motif protein 7) is an RNA-binding protein initially described to associate with splicing factors, presumably to target intronic RNA for decay (PMID: 35688134). RBM7 regulates the nuclear degradation of NEAT1 non-coding RNA, resulting in sustained apoptosis in the lung epithelium and fibrosis (PMID: 32708315).