RBM8A,Y14 Monoklonaler Antikörper

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• KD/KO Validated

RBM8A,Y14 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F5G1

Kat-Nr. : 67541-1-PBS

Synonyme

RBM8A, BOV 1C, BOV 1B, BOV 1A, BOV 1

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  WB analysis using 67541-1-Ig (same clone as 67541-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67541-1-Ig (RBM8A,Y14 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67541-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67541-1-Ig (same clone as 67541-1-PBS)

  WB result of RBM8A,Y14 antibody (67541-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells. This data was developed using the same antibody clone with 67541-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67541-1-Ig (same clone as 67541-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67541-1-Ig (RBM8A,Y14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67541-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 67541-1-Ig (same clone as 67541-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67541-1-Ig (RBM8A,Y14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67541-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67541-1-Ig (same clone as 67541-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67541-1-Ig (RBM8A,Y14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67541-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 67541-1-Ig (same clone as 67541-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using RBM8A,Y14 antibody (67541-1-Ig, Clone: 1F5G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67541-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67541-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RBM8A,Y14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBM8A,Y14 fusion protein Ag6800
• Vollständiger Name: RNA binding motif protein 8A
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC017088
• Gene symbol: RBM8A
• Gene ID (NCBI): 9939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.