FOX2/RBM9 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

FOX2/RBM9 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D7G5

Kat-Nr. : 66976-1-Ig

Synonyme

dJ106I20.3, Fox 1 homolog B, Fox 2, FOX2, FOX2/RBM9, fxh, HNRBP2, HRNBP2, RBFOX2, RBM9, RNA binding motif protein 9, RNA binding protein 9, RTA

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66976-1-Ig

  WB result of RBM9 antibody (66976-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM9 transfected HEK-293 cells.

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  WB analysis using 66976-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HT-29 using 66976-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 66976-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66976-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 66976-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66976-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66976-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66976-1-Ig (FOX2/RBM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66976-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66976-1-Ig (FOX2/RBM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66976-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66976-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66976-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66976-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66976-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, fetales humanes Hirngewebe, HEK-293-Zellen, HepG2-Zellen, HT-29-Zellen, humanes Plazenta-Gewebe, L02-Zellen, NCCIT-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Lungenkarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66976-1-Ig bindet in WB, IHC, ELISA FOX2/RBM9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FOX2/RBM9 fusion protein Ag28336
• Vollständiger Name: RNA binding motif protein 9
• Berechnetes Molekulargewicht: 390 aa, 41 kDa
• Beobachtetes Molekulargewicht: 48 kDa, 50 and 60 kDa
• GenBank-Zugangsnummer: BC013115
• Gene symbol: RBM9
• Gene ID (NCBI): 23543
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)