RBMS1 Rekombinanter Antikörper

RBMS1 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240677D6

Kat-Nr. : 83623-5-RR

Synonyme

MSSP 2, MSSP 1, MSSP, HCC 4, C2orf12

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  WB analysis using 83623-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83623-5-RR (RBMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 83623-5-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBMS1 Recombinant antibody (83623-5-RR, Clone:240677D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83623-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83623-5-RR against Human RBMS1 were performed. The affinity constant is 0.726 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83623-5-RR bindet in WB, FC (Intra), ELISA RBMS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RBMS1 fusion protein Ag1493
• Vollständiger Name: RNA binding motif, single stranded interacting protein 1
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC018951
• Gene symbol: RBMS1
• Gene ID (NCBI): 5937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBMS1 (RNA binding motif single stranded interacting protein 1), also known as C2orf12. It is expected to be located in the nucleus, which is ubiquitinated in placenta and endometrium. Highest amounts are found in placenta, lung and heart. This gene encodes a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. The molecular weight of RBMS1 is 45 kDa.