RBP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RBP2 Polyklonal Antikörper für WB, IF/ICC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18825-1-PBS

Synonyme

KDM5A, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, EC:1.14.11.67, JARID1A, Jumonji/ARID domain-containing protein 1A

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  WB analysis of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18825-1-AP (RBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 18825-1-AP (same clone as 18825-1-PBS)

  WB result of KDM5A antibody (18825-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and KDM5A knockout HEK-293T cells. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

  IP result of anti-RBP2 (IP:18825-1-AP, 4ug; Detection:18825-1-AP 1:500) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 18825-1-AP (same clone as 18825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 18825-1-AP (same clone as 18825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18825-1-AP (RBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18825-1-PBS bindet in WB, IF/ICC, IF-P, IP, Indirect ELISA RBP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: lysine (K)-specific demethylase 5A
• Berechnetes Molekulargewicht: 192 kDa
• Beobachtetes Molekulargewicht: 192 kDa
• GenBank-Zugangsnummer: NM_005056
• Gene symbol: KDM5A
• Gene ID (NCBI): 5927
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KDM5A, also named as JARID1A, RBBP2 and RBP2, belongs to the JARID1 histone demethylase family. KDM5A is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. RBP2 may stimulate transcription mediated by nuclear receptors. It may be involved in transcriptional regulation of Hox proteins during cell differentiation. It may participate in transcriptional repression of cytokines such as CXCL12.