RBP4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RBP4 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Rezensionen (1)

Kat-Nr. : 11774-1-AP

Synonyme

Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4

---

Galerie der Validierungsdaten

• 

  WB analysis using 11774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11774-1-AP (RBP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11774-1-AP

  WB result of RBP4 antibody (11774-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBP4 transfected HepG2 cells.

• 

  WB analysis of rat liver using 11774-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11774-1-AP (RBP4 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11774-1-AP (RBP4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11774-1-AP (RBP4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse eye using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11774-1-AP (RBP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11774-1-AP (RBP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11774-1-AP (RBP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11774-1-AP (RBP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11774-1-AP (RBP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 11774-1-AP (RBP4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 11774-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 11774-1-AP (RBP4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse eye using 11774-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RBP4 antibody (11774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human blood, HepG2-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Maus-Augengewebe, humanes Leberkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

11774-1-AP bindet in WB, IHC, IF, CoIP, ELISA RBP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Gänse, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBP4 fusion protein Ag2448
• Vollständiger Name: retinol binding protein 4, plasma
• Berechnetes Molekulargewicht: 201 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC020633
• Gene symbol: RBP4
• Gene ID (NCBI): 5950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)

Publikationen

Species	Application	Title
	WB	J Neuroinflammation; Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization; Authors - Tianyi Shen
mouse	WB,IF	Bioact Mater; Bio-inspired chiral self-assemblies promoted neuronal differentiation of retinal progenitor cells through activation of metabolic pathway.; Authors - Na Sun
mouse,human	WB	Theranostics; Growth hormone receptor disrupts glucose homeostasis via promoting and stabilizing retinol binding protein 4.; Authors - Jinxin Liu
mouse	IF	Int J Mol Sci; Retinoid Regulation of Ocular Surface Innate Inflammation.; Authors - Jehan Alam
mouse	WB,IHC	Metabolism; Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.; Authors - Shyi-Jang Shin
mouse	IHC	Virulence; Altering retinol binding protein 4 levels in hepatitis C: Inflammation and steatosis matter.; Authors - Ming-Ling Chang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Azita (Verified Customer) (05-31-2021)

Human spinal cord (cervical) tissue In 1/100 dilution gave fade labelling

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Human spinal cord (cervical) tissue

RBP4 Antibody Immunohistochemistry validation (1/100 dilution) in Human spinal cord (cervical) tissue (Cat no:11774-1-AP)