RBP4 Monoklonaler Antikörper

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• KD/KO Validated

RBP4 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D12B11

Kat-Nr. : 66104-1-PBS

Synonyme

1D12B11, Plasma retinol binding protein, Plasma retinol-binding protein, Plasma retinol-binding protein(1-176), Plasma retinol-binding protein(1-179)

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  WB analysis of human plasma using 66104-1-Ig (same clone as 66104-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66104-1-Ig (RBP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66104-1-Ig (same clone as 66104-1-PBS)

  WB result of RBP4 antibody (66104-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBP4 transfected HepG2 cells. This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66104-1-Ig (same clone as 66104-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66104-1-Ig (same clone as 66104-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66104-1-Ig (same clone as 66104-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66104-1-Ig (same clone as 66104-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66104-1-Ig (RBP4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66104-1-Ig (same clone as 66104-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using RBP4 antibody (66104-1-Ig, Clone: 1D12B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66104-1-Ig (same clone as 66104-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RBP4 antibody (66104-1-Ig, Clone: 1D12B11 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66104-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66104-1-PBS bindet in WB, IHC, IF/ICC, IF-P, ELISA RBP4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBP4 fusion protein Ag19295
• Vollständiger Name: retinol binding protein 4, plasma
• Berechnetes Molekulargewicht: 201 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC020633
• Gene symbol: RBP4
• Gene ID (NCBI): 5950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)