RBP4 Rekombinanter Antikörper

RBP4 Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241944B3

Kat-Nr. : 84496-1-PBS

Synonyme

241944B3, Plasma retinol binding protein, Plasma retinol-binding protein, Plasma retinol-binding protein(1-176), Plasma retinol-binding protein(1-179)

---

• 

  WB analysis of HepG2 using 84496-1-RR (same clone as 84496-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 84496-1-RR (RBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84496-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01338-1

  Sandwich ELISA standard curve of MP01338-1, Human RBP4 Recombinant Matched Antibody Pair - PBS only. 84496-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31642. 84496-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

• 

  Sample test of MP01338-1

  Serum of twelve individual healthy human donors was measured. The RBP4 concentration of detected samples was determined to be 87,804.85 ng/mL with a range of 37,160.99 - 156,178.64 ng/mL.

• 

  Sample test of MP01338-1

  Urine of six individual healthy human donors was measured. The RBP4 concentration of detected samples was determined to be 90.68 ng/mL with a range of 29.96 - 290.83 ng/mL.

• 

  Sample test of MP01338-1

  Saliva of six individual healthy human donors was measured. The RBP4 concentration of detected samples was determined to be 12.14 ng/mL with a range of 8.34-17.17 ng/mL.

• 

  Sample test of MP01338-1

  HepG2 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human RBP4, and measured 707.54 ng/mL.

• 

  FC experiment of HepG2 using 84496-1-RR (same clone as 84496-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBP4 Recombinant antibody (84496-1-RR, Clone:241944B3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84496-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01338-1

  Cytometric bead array standard curve of MP01338-1, RBP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84496-1-PBS. Detection antibody: 84496-2-PBS. Standard: Eg31642. Range: 0.156-20 ng/mL.

• 

  Affinity and Kinetic Characterization of 84496-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 84496-1-PBS against Human RBP4 were performed. The affinity constant is 0.504 nM.

• 

  Affinity and Kinetic Characterization of 84496-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84496-1-RR against Human RBP4 were performed. The affinity constant is 0.504 nM.

---

Geprüfte Anwendungen

Produktinformation

84496-1-PBS bindet in WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test RBP4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RBP4 fusion protein Eg31642
• Vollständiger Name: retinol binding protein 4, plasma
• Berechnetes Molekulargewicht: 201 aa, 23 kDa
• Beobachtetes Molekulargewicht: 20-21 kDa
• GenBank-Zugangsnummer: BC020633
• Gene symbol: RBP4
• Gene ID (NCBI): 5950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)