RBP4 Rekombinanter Antikörper

RBP4 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242040D6

Kat-Nr. : 84503-4-PBS

Synonyme

242040D6, Plasma retinol-binding protein, PRBP, RBP, Retinol-binding protein 4

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  WB analysis using 84503-4-RR (same clone as 84503-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84503-4-RR (RBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84503-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84503-4-RR (same clone as 84503-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84503-4-RR (RBP4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84503-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84503-4-RR (same clone as 84503-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84503-4-RR (RBP4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84503-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 84503-4-RR (same clone as 84503-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using RBP4 antibody (84503-4-RR, Clone: 242040D6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84503-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84503-4-PBS bindet in WB, IHC, IF-P, Indirect ELISA RBP4 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RBP4 fusion protein Eg1140
• Vollständiger Name: retinol binding protein 4, plasma
• Berechnetes Molekulargewicht: 23kDa
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: NM_011255.3
• Gene symbol: Rbp4
• Gene ID (NCBI): 19662
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)