RBP4 Monoklonaler Antikörper

RBP4 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1D12B11

Kat-Nr. : CL594-66104

Synonyme

Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4

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Galerie der Validierungsdaten

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  IF Staining of human liver cancer using CL594-66104

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 RBP4 antibody (CL594-66104, Clone: 1D12B11 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-66104

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 RBP4 antibody (CL594-66104, Clone: 1D12B11 ) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen, humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66104 bindet in IF RBP4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBP4 fusion protein Ag19295
• Vollständiger Name: retinol binding protein 4, plasma
• Berechnetes Molekulargewicht: 201 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC020633
• Gene symbol: RBP4
• Gene ID (NCBI): 5950
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)