RBP5 Monoklonaler Antikörper

RBP5 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G7E2

Kat-Nr. : 68126-1-Ig

Synonyme

CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5

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Galerie der Validierungsdaten

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  WB analysis of pig kidney using 68126-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 68126-1-Ig (RBP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 68126-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68126-1-Ig (RBP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68126-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68126-1-Ig (RBP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68126-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RBP5 antibody (68126-1-Ig, Clone: 1G7E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of A431 using 68126-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using RBP5 antibody (68126-1-Ig, Clone: 1G7E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Nierengewebe, Hausschwein-Lebergewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68126-1-Ig bindet in WB, IF, ELISA RBP5 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RBP5 fusion protein Ag24562
• Vollständiger Name: retinol binding protein 5, cellular
• Berechnetes Molekulargewicht: 135 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16-18 kDa
• GenBank-Zugangsnummer: BC029355
• Gene symbol: RBP5
• Gene ID (NCBI): 83758
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBP5 (CRBP III) belongs to the fatty-acid binding protein (FABP) family. Human liver and kidney contain the highest levels of CRBP III mRNA, and CRBP III is as a major human intracellular carrier of retinol (PMID:11274389). RBP5 iron response are reversible by genetic complementation. And increased RBP5 expression occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. RBP5 is the most significant of the proteins upregulated by iron starvation (PMID:34161395). RBP5 has an estimated molecular mass (15.9 kDa) typical of this protein superfamily (PMID:11274389).