RBPJ Polyklonaler Antikörper

RBPJ Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30044-1-PBS

Synonyme

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  WB analysis of MCF-7 using 30044-1-AP (same clone as 30044-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 30044-1-AP (same clone as 30044-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 30044-1-AP (same clone as 30044-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 30044-1-AP (same clone as 30044-1-PBS)

  IP result of anti-RBPJ (IP:30044-1-AP, 4ug; Detection:30044-1-AP 1:1000) with HepG2 cells lysate 1080 ug. This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 30044-1-AP (same clone as 30044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30044-1-AP (RBPJ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 30044-1-AP (same clone as 30044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30044-1-AP (RBPJ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30044-1-PBS bindet in WB, IHC, IP, Indirect ELISA RBPJ und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBPJ fusion protein Ag32552
• Vollständiger Name: recombination signal binding protein for immunoglobulin kappa J region
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: ~60 kDa
• GenBank-Zugangsnummer: BC064976
• Gene symbol: RBPJ
• Gene ID (NCBI): 3516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.