RBPJ Rekombinanter Antikörper

RBPJ Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243044G4

Kat-Nr. : 85676-3-RR

Synonyme

CBF 1, CBF1, CBF-1, csl, IGKJRB

---

• 

  WB analysis using 85676-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85676-3-RR (RBPJ antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85676-3-RR bindet in WB, ELISA RBPJ und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RBPJ fusion protein Ag32552
• Vollständiger Name: recombination signal binding protein for immunoglobulin kappa J region
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: ~60 kDa
• GenBank-Zugangsnummer: BC064976
• Gene symbol: RBPJ
• Gene ID (NCBI): 3516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.