RBPMS Rekombinanter Antikörper

RBPMS Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242730B7

Kat-Nr. : 85086-5-PBS

Synonyme

242730B7, Hermes, RBP MS, RBP-MS, RNA-binding protein with multiple splicing

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  WB analysis using 85086-5-RR (same clone as 85086-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85086-5-RR (RBPMS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 85086-5-RR (same clone as 85086-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85086-5-RR (RBPMS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 85086-5-RR (same clone as 85086-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85086-5-RR (RBPMS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse retina using 85086-5-RR (same clone as 85086-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse retina tissue using RBPMS antibody (85086-5-RR, Clone: 242730B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85086-5-RR (same clone as 85086-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBPMS antibody (85086-5-RR, Clone: 242730B7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 85086-5-RR (same clone as 85086-5-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug RBPMS Recombinant antibody (85086-5-RR, Clone:242730B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85086-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85086-5-RR against Human RBPMS were performed. The affinity constant is 0.153 nM.

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Geprüfte Anwendungen

Produktinformation

85086-5-PBS bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA RBPMS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RBPMS fusion protein Ag7256
• Vollständiger Name: RNA binding protein with multiple splicing
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC003608
• Gene symbol: RBPMS
• Gene ID (NCBI): 11030
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBPMS is a member of the RNA recognition motif family of RNA-binding proteins.It has a single, putative RNA recognition motif in its N-terminus.