RC3H1 Polyklonaler Antikörper

RC3H1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31234-1-PBS

Synonyme

KIAA2025, RING finger protein 198, RNF198, ROQUIN

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  WB analysis using 31234-1-AP (same clone as 31234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31234-1-AP (RC3H1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 31234-1-AP (same clone as 31234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 31234-1-AP (same clone as 31234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 31234-1-AP (same clone as 31234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 31234-1-AP (same clone as 31234-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RC3H1 antibody (31234-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31234-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RC3H1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RC3H1 fusion protein Ag35089
• Vollständiger Name: ring finger and CCCH-type zinc finger domains 1
• Berechnetes Molekulargewicht: 126 kDa
• Beobachtetes Molekulargewicht: 130-150 kDa
• GenBank-Zugangsnummer: NM_001300850
• Gene symbol: RC3H1
• Gene ID (NCBI): 149041
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Roquin-1 (Rc3h1) is an E3 ubiquitin ligase originally discovered in a mutational screen for genetic factors contributory to systemic lupus erythematosus-like symptoms in mice. A single base pair mutation in the Rc3h1 gene resulted in the manifestation of autoantibody production and sustained immunological inflammation characterized by excessive T follicular helper cell activation and formation of germinal centers. (PMID: 26673963)