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RCC1 Monoklonaler Antikörper

RCC1 Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2C2F5

Kat-Nr. : CL594-67335

Synonyme

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated RCC1 Monoclonal antibody (CL594-67335)

    IF Staining of HepG2 using CL594-67335

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RCC1 antibody (CL594-67335, Clone: 2C2F5 ) at dilution of 1:200. DAPI (blue).

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67335 bindet in IF/ICC RCC1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen RCC1 fusion protein Ag17809
Vollständiger Name regulator of chromosome condensation 1
Berechnetes Molekulargewicht 421 aa, 45 kDa
Beobachtetes Molekulargewicht 50 kDa
GenBank-ZugangsnummerBC010067
Gene symbol RCC1
Gene ID (NCBI) 1104
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL594 RCC1 antibody CL594-67335Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen