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  • Featured Product
  • KD/KO Validated

Pirh2 Polyklonaler Antikörper

Pirh2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13820-1-AP

Synonyme

ARNIP, CHIMP, hARNIP, hPirh2, p53 induced RING H2 protein, Pirh2, RCHY1, RING finger protein 199, RNF199, Zinc finger protein 363, ZNF363

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using Pirh2 Polyclonal antibody (13820-1-AP)

    WB analysis using 13820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13820-1-AP (Pirh2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Pirh2 Polyclonal antibody (13820-1-AP)

    WB analysis of HEK-293 using 13820-1-AP

    WB result of Pirh2 antibody (13820-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pirh2 transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Pirh2 Polyclonal antibody (13820-1-AP)

    IHC staining of mouse liver using 13820-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13820-1-AP (Pirh2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Pirh2 Polyclonal antibody (13820-1-AP)

    IHC staining of mouse liver using 13820-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13820-1-AP (Pirh2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausmilzgewebe, HEK-293-Zellen, Maushodengewebe
Erfolgreiche Detektion in IHCMauslebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13820-1-AP bindet in WB, IHC, ELISA Pirh2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Pirh2 fusion protein Ag4916
Vollständiger Name ring finger and CHY zinc finger domain containing 1
Berechnetes Molekulargewicht 261 aa, 30 kDa
Beobachtetes Molekulargewicht 45 KDa, 30 kDa
GenBank-ZugangsnummerBC047393
Gene symbol RCHY1
Gene ID (NCBI) 25898
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.

Protokolle

Produktspezifische Protokolle
WB protocol for Pirh2 antibody 13820-1-APProtokoll herunterladen
IHC protocol for Pirh2 antibody 13820-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Sci Adv

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Authors - Pengbo Cao
  • KD Validated
View Article
humanWB,IHC

Sci Adv

G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.

Authors - Yang Zhang
View Article
humanWB

J Agric Food Chem

Avenanthramide A induces cellular senescence via miR-129-3p/Pirh2/p53 signaling pathway to suppress colon cancer growth.

Authors - Rong Fu
  • KD Validated
View Article