RCN1 Rekombinanter Antikörper

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• KD/KO Validated

RCN1 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240473E9

Kat-Nr. : 83638-3-PBS

Synonyme

Reticulocalbin 1, 240473E9, PIG20, RCAL, RCN

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  WB analysis using 83638-3-RR (same clone as 83638-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83638-3-RR (RCN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

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  WB analysis using 83638-3-RR (same clone as 83638-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83638-3-RR (RCN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83638-3-RR (same clone as 83638-3-PBS)

  WB result of RCN1 antibody (83638-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCN1 transfected HeLa cells. This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83638-3-RR (same clone as 83638-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RCN1 antibody (83638-3-RR, Clone: 240473E9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83638-3-RR (same clone as 83638-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RCN1 Recombinant antibody (83638-3-RR, Clone:240473E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83638-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00637-3

  Cytometric bead array standard curve of MP00637-3, RCN1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83638-4-PBS. Detection antibody: 83638-3-PBS. Standard: Ag13256. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83638-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83638-3-PBS against Human RCN1 was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 83638-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83638-3-RR against Human RCN1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83638-3-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA RCN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RCN1 fusion protein Ag13256
• Vollständiger Name: reticulocalbin 1, EF-hand calcium binding domain
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 44-46 kDa
• GenBank-Zugangsnummer: BC010120
• Gene symbol: RCN1
• Gene ID (NCBI): 5954
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Reticulocalbin-1 (RCN1) belongs to the CREC family. Reticulocalbin‐1, an endoplasmic reticulum protein, plays a crucial role in calcium homeostasis and inhibits ER stress‐induced apoptosis (PMID: 28319095). Its main functions include maintaining intracellular homeostasis and regulating cell proliferation and apoptosis, and it plays an important role in the development of various tumours (PMID: 38057406). RCN1 is expressed aberrantly and at a high level in various tumors, including acute myeloid leukemia (AML) (PMID: 37746742). In multiple cancers, such as glioblastoma, non‐small cell lung cancer, renal cell carcinoma, hepatocellular carcinoma, and oral squamous cell carcinoma, the overexpression of RCN1 has been observed indicating its involvement in tumorigenesis and invasion (PMID: 30172915, PMID: 34722631).