RCN2 Polyklonaler Antikörper

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• KD/KO Validated

RCN2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10193-2-PBS

Synonyme

Calcium binding protein ERC 55, Calcium-binding protein ERC-55, E6 binding protein, E6-binding protein, E6BP

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  WB analysis using 10193-2-AP (same clone as 10193-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10193-2-AP (same clone as 10193-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10193-2-AP (same clone as 10193-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 10193-2-AP (same clone as 10193-2-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 10193-2-AP (same clone as 10193-2-PBS)

  IP result of anti-RCN2 (IP:10193-2-AP, 4ug; Detection:10193-2-AP 1:400) with mouse lung tissue lysate 2800ug. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 10193-2-AP (same clone as 10193-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 10193-2-AP (same clone as 10193-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 10193-2-AP (same clone as 10193-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 10193-2-AP (same clone as 10193-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10193-2-AP (same clone as 10193-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 10193-2-AP (same clone as 10193-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10193-2-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA RCN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RCN2 fusion protein Ag0247
• Vollständiger Name: reticulocalbin 2, EF-hand calcium binding domain
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC004892
• Gene symbol: RCN2
• Gene ID (NCBI): 5955
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.

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