RCOR1 Polyklonaler Antikörper

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• KD/KO Validated

RCOR1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 27686-1-AP

Synonyme

RCOR 1, RCOR, COREST

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  WB analysis of C2C12 using 27686-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 27686-1-AP (RCOR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 27686-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 27686-1-AP (RCOR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27686-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27686-1-AP (RCOR1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12 cell, PC-12-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

27686-1-AP bindet in WB, IHC, IF, ELISA RCOR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RCOR1 fusion protein Ag26770
• Vollständiger Name: REST corepressor 1
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: NM_015156
• Gene symbol: RCOR1
• Gene ID (NCBI): 23186
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RCOR1, also named as REST corepressor 1, is a 485 amino acid protein, which belongs to the CoREST family. RCOR1 is a essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. The calculated molecular weight of RCOR1 is a 53 kDa, but the phosphorylated RCOR1 is about 60-65 kDa.

Publikationen

Species	Application	Title
human	WB	EMBO J; The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.; Authors - Buqing Ye
	WB,IF	J Biol Chem; HKDC1 promotes colorectal cancer progression by regulating RCOR1 expression to activate the Wnt/β-catenin pathway, enhancing proliferation, migration, and epithelial-mesenchymal transition (EMT); Authors - Shansong Huang
rat	WB	Biochem Biophys Res Commun; Increased NRSF/REST in anterior cingulate cortex contributes to diabetes-related neuropathic pain.; Authors - Xu Xiao-Die
human	IHC	Ann Diagn Pathol; microRNA 155 up regulation in the CNS is strongly correlated to Down's syndrome dementia.; Authors - Esmerina Tili
human	WB	Nat Commun; Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition; Authors - Cheng Zeng ; KO Validated
human	WB	Cell Chem Biol; HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation; Authors - Yufeng Xiao