RCOR2 Polyklonaler Antikörper

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RCOR2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23969-1-PBS

Synonyme

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  WB analysis of Jurkat using 23969-1-AP (same clone as 23969-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23969-1-AP (RCOR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23969-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 23969-1-AP (same clone as 23969-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23969-1-AP (RCOR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23969-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 23969-1-AP (same clone as 23969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 23969-1-AP (RCOR2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23969-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 23969-1-AP (same clone as 23969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 23969-1-AP (RCOR2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23969-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 23969-1-AP (same clone as 23969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 23969-1-AP (RCOR2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23969-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23969-1-PBS bindet in WB, IHC, Indirect ELISA RCOR2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RCOR2 fusion protein Ag21112
• Vollständiger Name: REST corepressor 2
• Berechnetes Molekulargewicht: 523 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC023587
• Gene symbol: RCOR2
• Gene ID (NCBI): 283248
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In mammals, the CoREST (corepressor for element-1-silencing transcription factor) complex is a chromatin-modifying structure that, through interactions with NRSF (neuron restrictive silencer factor), regulates neuronal gene expression and neuronal cell fate. RCOR2 (REST corepressor 2) and RCOR3 (REST corepressor 3) are nuclear proteins that each contain one ELM2 domain and two SANT domains. RCOR2 and RCOR3, both members of the CoREST family, are thought to function as components of the CoREST complex, possibly playing a role in the transcriptional repression of neuronal genes. Additionally, RCOR2 and RCOR3, in conjunction with CoREST, can form immunocomplexes with a variety of histone-modifying genes, including G9a and HDAC1. Via these protein complexes, RCOR2 and RCOR3 can further regulate transcription by controlling the methylation and demethylation of target genes during early development.