RD3 Polyklonaler Antikörper

RD3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14855-1-PBS

Synonyme

C1orf36, LCA12, Protein RD3, retinal degeneration 3, Retinal degeneration protein 3

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  WB analysis using 14855-1-AP (same clone as 14855-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14855-1-AP (RD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 14855-1-AP (same clone as 14855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

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  IHC staining of rat eye using 14855-1-AP (same clone as 14855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

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  IHC staining of rat eye using 14855-1-AP (same clone as 14855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 14855-1-AP (same clone as 14855-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using RD3 antibody (14855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14855-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RD3 fusion protein Ag6641
• Vollständiger Name: retinal degeneration 3
• Berechnetes Molekulargewicht: 22.7 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC065541
• Gene symbol: RD3
• Gene ID (NCBI): 343035
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RD3, or Retinal Degeneration 3, plays a critical role in regulating guanylate cyclase (GC) signaling and photoreceptor cell survival (PMID: 30559291). RD3 is highly conserved across vertebrates, with the human protein sharing high sequence identity with other primates and varying degrees of identity with other species (PMID: 29030614). The main functions of RD3 include inhibiting photoreceptor-specific guanylate cyclase activity and promoting the accumulation of retinal membrane guanylyl cyclase (RetGC) in the photoreceptor outer segment (PMID: 30559291). RD3 is essential for the normal expression of RetGC in photoreceptor cells and blocks RetGC catalytic activity. Mutations in the RD3 gene can lead to Leber congenital amaurosis type 12, which results in retinal degeneration. RD3 is also involved in the trafficking of RetGC from the endoplasmic reticulum to the photoreceptor outer segments, which is crucial for maintaining the normal function and survival of photoreceptors (PMID: 34537244).