NELFE Polyklonaler Antikörper

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• KD/KO Validated

NELFE Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Rezensionen (1)

Kat-Nr. : 10705-1-AP

Synonyme

NELF E, NELF-E, RD, RDBP, RNA-binding protein RD

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  WB analysis of HEK-293 using 10705-1-AP

  WB result of NELFE antibody (10705-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NELFE transfected HEK-293 cells.

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  WB analysis using 10705-1-AP

  Various lyastes were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 10705-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 10705-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10705-1-AP

  IP result of anti-NELFE (IP:10705-1-AP, 4ug; Detection:10705-1-AP 1:6000) with mouse brain tissue lysate 1840 ug.

• 

  IHC staining of human lung cancer using 10705-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10705-1-AP (NELFE antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10705-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10705-1-AP (NELFE antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NELFE antibody (10705-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of hela using 10705-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human NELFE (10705-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug rabbit IgG isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Maus-Augengewebe, Mausnierengewebe, Maus-Plazenta-Gewebe, Rattenhirngewebe, Rattennierengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• ChIP: See 4 publications below
• RIP: See 1 publications below

Produktinformation

10705-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA NELFE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NELFE fusion protein Ag1186
• Vollständiger Name: RD RNA binding protein
• Berechnetes Molekulargewicht: 37 kDa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC011600
• Gene symbol: NELFE
• Gene ID (NCBI): 7936
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

Publikationen

Species	Application	Title
mouse	IF	Nature; The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.; Authors - Bofeng Liu
mouse,human	WB	Cell; Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.; Authors - Sawarkar Ritwick R
mouse	WB,chIP	Nat Commun; Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.; Authors - Li Yu
mouse	WB	Nat Commun; RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity.; Authors - Bogang Wu
mouse	WB	Dev Cell; Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.; Authors - Daniel C L Robinson
mouse	WB,IHC	Proc Natl Acad Sci U S A; Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.; Authors - Adelman Karen K

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

HUAI-CHIN (Verified Customer) (02-14-2019)

This antibody works great in Western. Gives me clean single band.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse embryonic fibroblast