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RDBP Rekombinanter Antikörper

RDBP Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250420G2

Kat-Nr. : 85945-1-PBS

Synonyme

NELFE, NELF E, NELF-E, RD, RNA-binding protein RD

Filter:
  • Western Blot (WB) analysis of various lysates using RDBP Recombinant antibody (85945-1-RR)

    WB analysis using 85945-1-RR (same clone as 85945-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RDBP Recombinant antibody (85945-1-RR)

    WB analysis using 85945-1-RR (same clone as 85945-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RDBP Recombinant antibody (85945-1-RR)

    IF Staining of HeLa using 85945-1-RR (same clone as 85945-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RDBP antibody (85945-1-RR, Clone: 250420G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85945-1-PBS bindet in WB, IF/ICC, Indirect ELISA RDBP und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RDBP fusion protein Ag1186
Vollständiger Name RD RNA binding protein
Berechnetes Molekulargewicht 37 kDa, 43 kDa
Beobachtetes Molekulargewicht42 kDa
GenBank-ZugangsnummerBC011600
Gene symbol NELFE
Gene ID (NCBI) 7936
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.