Radixin Polyklonaler Antikörper

Radixin Polyklonal Antikörper für FC, IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13790-1-AP

Synonyme

DFNB24, radixin, RDX

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Galerie der Validierungsdaten

• 

  WB analysis of mouse pancreas using 13790-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 13790-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13790-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 13790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13790-1-AP (Radixin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 13790-1-AP

  Immunofluorescent analysis of MCF-7 cells using 13790-1-AP (Radixin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 13790-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Radixin antibody (13790-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, HEK-293-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Leberkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

13790-1-AP bindet in WB, IHC, IF, FC,ELISA Radixin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Radixin fusion protein Ag4813
• Vollständiger Name: radixin
• Berechnetes Molekulargewicht: 583 aa, 68 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC047109
• Gene symbol: RDX
• Gene ID (NCBI): 5962
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Radixin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to chemokine and antigen stimulation.

Publikationen

Species	Application	Title
mouse	IF	Am J Pathol; Elevated Expression of Moesin in Muscular Dystrophies.; Authors - Mark Pines