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RECQL Rekombinanter Antikörper

RECQL Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

242577F1

Kat-Nr. : CL594-85024-6

Synonyme

242577F1, ATP dependent DNA helicase Q1, ATP-dependent DNA helicase Q1, DNA 3'-5' helicase Q1, DNA dependent ATPase Q1

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated RECQL Recombinant antibody (CL594-85024-6)

    IF Staining of HeLa using CL594-85024-6

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 RECQL antibody (CL594-85024-6, Clone: 242577F1 ) at dilution of 1:200, CL488-Phalloidin (green).

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-85024-6 bindet in IF/ICC RECQL und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RECQL fusion protein Ag6834
Vollständiger Name RecQ protein-like (DNA helicase Q1-like)
Berechnetes Molekulargewicht 73 kDa
GenBank-ZugangsnummerBC001052
Gene symbol RECQL
Gene ID (NCBI) 5965
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RECQL, also known as RecQ-like helicase, is a member of the RecQ DNA helicase family. These enzymes play a crucial role in maintaining genomic integrity by participating in various DNA repair processes, including mismatch repair, nucleotide excision repair, and direct repair. It's reported that the low RECQL expression is strongly associated with poor prognosis in breast cancer. RECQL expression may be a useful marker in estimating the prognosis of breast cancer patients. (PMID: 29914420)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL594 RECQL antibody CL594-85024-6Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen