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REDD1 Monoklonaler Antikörper

REDD1 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A2C10

Kat-Nr. : 67059-1-PBS

Synonyme

DDIT4, 3A2C10, DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, REDD-1

Filter:
  • Western Blot (WB) analysis of various lysates using REDD1 Monoclonal antibody (67059-1-Ig)

    WB analysis using 67059-1-Ig (same clone as 67059-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67059-1-Ig (REDD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67059-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using REDD1 Monoclonal antibody (67059-1-Ig)

    WB analysis of HeLa using 67059-1-Ig (same clone as 67059-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67059-1-Ig (REDD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67059-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67059-1-PBS bindet in WB, Indirect ELISA REDD1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen REDD1 fusion protein Ag0965
Vollständiger Name DNA-damage-inducible transcript 4
Berechnetes Molekulargewicht 25 kDa
Beobachtetes Molekulargewicht 32-35 kDa
GenBank-ZugangsnummerBC007714
Gene symbol REDD1/DDIT4
Gene ID (NCBI) 54541
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489].