REDD1 Rekombinanter Antikörper

REDD1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1L2

Kat-Nr. : 82650-1-RR

Synonyme

DDIT4, 1L2, RTP801, REDD-1, HIF-1 responsive protein RTP801

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  WB analysis using 82650-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82650-1-RR (REDD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 82650-1-RR

  Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 82650-1-RR (REDD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82650-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82650-1-RR against Human REDD1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, mit Cobaltchlorid behandelte HeLa-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82650-1-RR bindet in WB, ELISA REDD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: REDD1 fusion protein Ag0965
• Vollständiger Name: DNA-damage-inducible transcript 4
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: BC007714
• Gene symbol: REDD1/DDIT4
• Gene ID (NCBI): 54541
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489].