REEP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

REEP1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 17988-1-AP

Synonyme

C2orf23, receptor accessory protein 1, REEP1, SPG31

---

• 

  WB analysis using 17988-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17988-1-AP (REEP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17988-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17988-1-AP (REEP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17988-1-AP

  IP result of anti-REEP1 (IP:17988-1-AP, 3ug; Detection:17988-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 17988-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17988-1-AP (REEP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17988-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17988-1-AP (REEP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

17988-1-AP bindet in WB, IHC, IF, IP, ELISA REEP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: REEP1 fusion protein Ag12418
• Vollständiger Name: receptor accessory protein 1
• Berechnetes Molekulargewicht: 201 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC064846
• Gene symbol: REEP1
• Gene ID (NCBI): 65055
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Struct Mol Biol; Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis; Authors - Wei Zhang
human,mouse	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
	IP	Mol Psychiatry; Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.; Authors - M G Sharoar
human	WB	Autophagy; DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.; Authors - Xiaoyu Tian
mouse	WB,IF	Hum Mol Genet; Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation.; Authors - Benoît Renvoisé ; KO Validated
human	WB	Neurobiol Dis; Disease-causing mutated ATLASTIN 3 is excluded from distal axons and reduces axonal autophagy.; Authors - Laura Behrendt