REEP2 Polyklonaler Antikörper

REEP2 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (1)

Kat-Nr. : 15684-1-AP

Synonyme

C5orf19, receptor accessory protein 2, REEP2, SGC32445

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Galerie der Validierungsdaten

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  WB analysis using 15684-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 15684-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 15684-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 15684-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 15684-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15684-1-AP (REEP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15684-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15684-1-AP (REEP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 15684-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 15684-1-AP (REEP2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 15684-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug REEP2 antibody (15684-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Maus-Augengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

15684-1-AP bindet in WB, IP, IHC, IF, FC, ELISA REEP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: REEP2 fusion protein Ag8186
• Vollständiger Name: receptor accessory protein 2
• Berechnetes Molekulargewicht: 252 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC006218
• Gene symbol: REEP2
• Gene ID (NCBI): 51308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
mouse	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
human,mouse	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
mouse	IP	Mol Psychiatry; Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.; Authors - M G Sharoar
mouse	WB	Hum Mol Genet; Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation.; Authors - Benoît Renvoisé
mouse	WB	Hum Mol Genet; Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.; Authors - Peng-Peng Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wanxia (Verified Customer) (07-16-2019)

Very good Reep2 antibody for WB and IF. It is so clean, almost no background.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: Mouse Brain

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