REEP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

REEP2 Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15684-1-PBS

Synonyme

---

• 

  WB analysis using 15684-1-AP (same clone as 15684-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse eye using 15684-1-AP (same clone as 15684-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 15684-1-AP (same clone as 15684-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 15684-1-AP (same clone as 15684-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15684-1-AP (same clone as 15684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15684-1-AP (REEP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15684-1-AP (same clone as 15684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15684-1-AP (REEP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 15684-1-AP (same clone as 15684-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 15684-1-AP (REEP2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 15684-1-AP (same clone as 15684-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug REEP2 Polyclonal antibody (15684-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

15684-1-PBS bindet in WB, IHC, IF-P, FC (Intra), Indirect ELISA REEP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: REEP2 fusion protein Ag8186
• Vollständiger Name: receptor accessory protein 2
• Berechnetes Molekulargewicht: 252 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC006218
• Gene symbol: REEP2
• Gene ID (NCBI): 51308
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.