REEP4 Rekombinanter Antikörper

REEP4 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250770C3

Kat-Nr. : 86164-2-RR

Synonyme

C8orf20, PP432, Receptor expression-enhancing protein 4

---

• 

  WB analysis using 86164-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86164-2-RR (REEP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86164-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86164-2-RR (REEP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 86164-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86164-2-RR (REEP4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 86164-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86164-2-RR against Human REEP4 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, BxPC-3-Zellen, HaCaT-Zellen, Maushodengewebe, Rattenhodengewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86164-2-RR bindet in WB, IHC, ELISA REEP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: REEP4 fusion protein Ag24569
• Vollständiger Name: receptor accessory protein 4
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC013048
• Gene symbol: REEP4
• Gene ID (NCBI): 80346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REEP4 (Receptor Expression-Enhancing Protein 4) is a member of the REEP family of proteins, a transmembrane protein that interacts with odorant receptor proteins and may enhance the odorant receptor responses to odorants. It is localized to the endoplasmic reticulum (ER) and is associated with the nuclear envelope (NE). REEP4 helps link the high-curvature ER to the ELYS-based nuclear pore complex (NPC) seed, thereby promoting NPC biogenesis during late mitosis. REEP4 expression is associated with the cell cycle, osteoclast differentiation, Fc gamma R-mediated phagocytosis, and Human T-cell leukemia virus 1 infection. (PMID: 36191880; 38947393)