REEP5 Monoklonaler Antikörper

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REEP5 Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H8D8

Kat-Nr. : 68119-1-PBS

Synonyme

1H8D8, C5orf18, DP1, Polyposis locus protein 1, Protein TB2

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  WB analysis using 68119-1-Ig (same clone as 68119-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68119-1-Ig (same clone as 68119-1-PBS)

  WB result of REEP5 antibody (68119-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REEP5 transfected HeLa cells. This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 68119-1-Ig (same clone as 68119-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68119-1-Ig (same clone as 68119-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68119-1-Ig (same clone as 68119-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68119-1-Ig (same clone as 68119-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 68119-1-Ig (same clone as 68119-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using REEP5 antibody (68119-1-Ig, Clone: 1H8D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50780-1

  Cytometric bead array standard curve of MP50780-1, REEP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68119-1-PBS. Detection antibody: 68119-2-PBS. Standard:Ag6229. Range: 0.098-100 ng/mL.

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  Cytometric bead array standard curve of MP50780-2

  Cytometric bead array standard curve of MP50780-2, REEP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68119-1-PBS. Detection antibody: 68119-3-PBS. Standard:Ag6229. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68119-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA REEP5 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: REEP5 fusion protein Ag6229
• Vollständiger Name: receptor accessory protein 5
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC065926
• Gene symbol: REEP5
• Gene ID (NCBI): 7905
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).