REL Monoklonaler Antikörper

REL Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1F6A4

Kat-Nr. : 67747-1-Ig

Synonyme

C Rel, Proto oncogene c Rel, REL

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  WB analysis using 67747-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67747-1-Ig (REL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of HEK-293 using 67747-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67747-1-Ig (REL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HEK-293-Zellen, Jurkat-Zellen, MOLT-4-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67747-1-Ig bindet in WB, ELISA REL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: REL fusion protein Ag26803
• Vollständiger Name: v-rel reticuloendotheliosis viral oncogene homolog (avian)
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 68 kDa, 80 kDa
• GenBank-Zugangsnummer: NM_001291746
• Gene symbol: REL
• Gene ID (NCBI): 5966
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REL, also known as, Proto-oncogene c-Rel, is a 619 amino acid protein, which may play a role in differentiation and lymphopoiesis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator. The full-length 80-kDa c-Rel was identified (PMID: 9857058).

Publikationen

Species	Application	Title
	WB	Invest Ophthalmol Vis Sci; Global MicroRNA Profiling of HSV-1 Infected Cornea Identifies miR-329 as a Novel Regulator of Virus Infection; Authors - Pankaj Sharma