RELN Polyklonaler Antikörper

RELN Polyklonal Antikörper für IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20689-1-PBS

Synonyme

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  IHC staining of human liver cancer using 20689-1-AP (same clone as 20689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20689-1-AP (RELN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20689-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 20689-1-AP (same clone as 20689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20689-1-AP (RELN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20689-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 20689-1-AP (same clone as 20689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20689-1-AP (RELN Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 20689-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 20689-1-AP (same clone as 20689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 20689-1-AP (RELN Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 20689-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 20689-1-AP (same clone as 20689-1-PBS)

  Dual immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20689-1-AP (RELN antibody) at dilution of 1:50 and 66564-1-Ig (Tbr1 antibody), further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 20689-1-AP and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66564-1-Ig. This data was developed using the same antibody clone with 20689-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 20689-1-AP (same clone as 20689-1-PBS)

  Dual immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20689-1-AP (RELN antibody) at dilution of 1:50 and 66564-1-Ig (Tbr1 antibody), further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 20689-1-AP and Alexa Fluor 594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66564-1-Ig. This data was developed using the same antibody clone with 20689-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20689-1-PBS bindet in IHC, IF-P, Indirect ELISA RELN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: reelin
• Berechnetes Molekulargewicht: 388 kDa
• GenBank-Zugangsnummer: NM_005045
• Gene symbol: RELN
• Gene ID (NCBI): 5649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RELN belongs to the reelin family. It is an extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. It is a secreted glycoprotein which is essential for neuronal migration in the cortex. RELN regulates microtubule function in neurons and neuronal migration. It affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and ApoER2, RELN induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. Defects in RELN are the cause of lissencephaly type 2 (LIS2).