RENALASE Polyklonaler Antikörper

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• KD/KO Validated

RENALASE Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 15003-1-AP

Synonyme

C10orf59, MAO C, Monoamine oxidase C, RENALASE, RNLS

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Galerie der Validierungsdaten

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  WB analysis using 15003-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15003-1-AP (RENALASE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15003-1-AP

  IP Result of anti-RENALASE (IP:15003-1-AP, 4ug; Detection:15003-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human kidney using 15003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15003-1-AP (RENALASE antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 15003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15003-1-AP (RENALASE antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of mouse kidney using 15003-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using RENALASE antibody (15003-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 15003-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 15003-1-AP (RENALASE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausnierengewebe, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

15003-1-AP bindet in WB, IP, IHC, IF, ELISA RENALASE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RENALASE fusion protein Ag13061
• Vollständiger Name: chromosome 10 open reading frame 59
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC005364
• Gene symbol: C10orf59
• Gene ID (NCBI): 55328
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.

Publikationen

Species	Application	Title
human,rat	WB,IHC	Circulation; Catecholamines regulate the activity, secretion, and synthesis of renalase.; Authors - Li Guoyong G
human,rat	WB,IHC	J Clin Invest; Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure.; Authors - Xu Jianchao J
mouse	WB	Kidney Int; Renalase deficiency aggravates ischemic myocardial damage.; Authors - Wu Yanling Y ; KO Validated
rat	WB,IF	Front Cardiovasc Med; Aerobic Exercise Training Improves Renal Injury in Spontaneously Hypertensive Rats by Increasing Renalase Expression in Medulla.; Authors - Minghao Luo
human	WB,IF	Life Sci; Renalase is localized to the small intestine crypt and expressed upon the activation of NF-κB p65 in mice model of fasting-induced oxidative stress.; Authors - Kai Aoki
human	WB	Exp Biol Med (Maywood); Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients.; Authors - Janete Quelhas-Santos