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  • KD/KO Validated

REST Polyklonaler Antikörper

REST Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(31)

Kat-Nr. : 22242-1-AP

Synonyme

XBR, X2 box repressor, NRSF



Geprüfte Anwendungen

Erfolgreiche Detektion in WBRaji-Zellen, HEK-293-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

22242-1-AP bindet in WB, IHC, IF, IP, CoIP, ChIP, ELISA REST und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, hamster, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen REST fusion protein Ag17707
Vollständiger Name RE1-silencing transcription factor
Berechnetes Molekulargewicht 1097 aa, 122 kDa
Beobachtetes Molekulargewicht 200 kDa
GenBank-ZugangsnummerBC132859
Gene symbol REST
Gene ID (NCBI) 5978
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for REST antibody 22242-1-APProtokoll herunterladen
IHC protocol for REST antibody 22242-1-APProtokoll herunterladenl
IP protocol for REST antibody 22242-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Sci Adv

EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency

Authors - Chikako Okubo
mouse

Sci Adv

YAP drives fate conversion and chemoresistance of small cell lung cancer.

Authors - Qingzhe Wu
  • KD Validated
humanWB

Cell Death Differ

The cross talk of two family members of β-TrCP in the regulation of cell autophagy and growth.

Authors - Danrui Cui
ratWB,IF

Oncotarget

REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration and neuronal death partially via LRP6-mediated Wnt-β-catenin signaling.

Authors - Zhiqi Song
  • KD Validated
humanWB,CoIP

Cell Death Dis

ALKBH5-mediated m 6 A demethylation of KCNK15-AS1 inhibits pancreatic cancer progression via regulating KCNK15 and PTEN/AKT signaling

Authors - Yuan He
hamsterWB,IF

Front Mol Neurosci

Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models.

Authors - Zhiqi Song

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Aktan (Verified Customer) (12-24-2019)

The product yield satisfactory results with 5%BSA in PBST blocking; primary dilution in the blocking buffer and o/n incubation. The blot is washed and incubated with Licor near IR dye-conjugated goat-antirabbit secondary antibodies for 1h and after washing the bands were detected in Licor.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Prostate cancer cell lines
REST Antibody Western Blot, validation (1:500 dilution) in Prostate cancer cell lines (Cat no:22242-1-AP)