REST Polyklonaler Antikörper

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REST Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22242-1-PBS

Synonyme

XBR, X2 box repressor, NRSF

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  WB analysis of Raji using 22242-1-AP (same clone as 22242-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 22242-1-AP (REST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 22242-1-AP (same clone as 22242-1-PBS)

  WB result of REST antibody (22242-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REST transfected HEK-293 cells. This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 22242-1-AP (same clone as 22242-1-PBS)

  IP result of anti-REST (IP:22242-1-AP, 4ug; Detection:22242-1-AP 1:15000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22242-1-AP (same clone as 22242-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22242-1-AP (same clone as 22242-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22242-1-PBS bindet in WB, IHC, IP, Indirect ELISA REST und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: REST fusion protein Ag17707
• Vollständiger Name: RE1-silencing transcription factor
• Berechnetes Molekulargewicht: 1097 aa, 122 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC132859
• Gene symbol: REST
• Gene ID (NCBI): 5978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).