REST Rekombinanter Antikörper

REST Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

241789D3

Kat-Nr. : 84846-4-PBS

Synonyme

241789D3, NRSF, X2 box repressor, XBR

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  WB analysis using 84846-4-RR (same clone as 84846-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84846-4-RR (REST antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84846-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84846-4-RR (same clone as 84846-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using REST antibody (84846-4-RR, Clone: 241789D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84846-4-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 84846-4-RR (same clone as 84846-4-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of REST (84846-4-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84846-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84846-4-PBS bindet in WB, IF/ICC, Indirect ELISA, ChIP-qPCR REST und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: REST fusion protein Ag17707
• Vollständiger Name: RE1-silencing transcription factor
• Berechnetes Molekulargewicht: 1097 aa, 122 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC132859
• Gene symbol: REST
• Gene ID (NCBI): 5978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as a transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected at 200 kDa by western blot in our result, which is similar to the article (PMID: 16764822).