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RFC1 Rekombinanter Antikörper

RFC1 Rekombinant Antikörper für WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242818H6

Kat-Nr. : 85148-4-PBS

Synonyme

A1, A1 140 kDa subunit, Activator 1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1

Filter:
  • Western Blot (WB) analysis of various lysates using RFC1 Recombinant antibody (85148-4-RR)

    WB analysis using 85148-4-RR (same clone as 85148-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85148-4-RR (RFC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85148-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01865-3

    Sandwich ELISA standard curve of MP01865-3

    Sandwich ELISA standard curve of MP01865-3, Human RFC1 Recombinant Matched Antibody Pair - PBS only. 85148-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag13585. 85148-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01865-3

    Sample test of MP01865-3

    The mean RFC1 concentration was determined to be 21.39 ng/mL in U2OS cell extract based on a 3.50 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of human colon tissue using RFC1 Recombinant antibody (85148-4-RR)

    IHC staining of human colon using 85148-4-RR (same clone as 85148-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85148-4-RR (RFC1 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85148-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RFC1 Recombinant antibody (85148-4-RR)

    IF Staining of HeLa using 85148-4-RR (same clone as 85148-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC1 antibody (85148-4-RR, Clone: 242818H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85148-4-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85148-4-PBS bindet in WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test RFC1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RFC1 fusion protein Ag13585
Vollständiger Name replication factor C (activator 1) 1, 145kDa
Berechnetes Molekulargewicht 128 kDa
Beobachtetes Molekulargewicht140 kDa
GenBank-ZugangsnummerBC051751
Gene symbol RFC1
Gene ID (NCBI) 5981
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Replication factor C subunit 1 (RFC1) is a pentameric DNA polymerase accessory protein involved in DNA replication and repair (PMID: 10051561). It was reported as a cause of cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS) (PMID: 30926972).