RFC2 Rekombinanter Antikörper

RFC2 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242503E12

Kat-Nr. : 84892-1-PBS

Synonyme

242503E12, A1, A1 40 kDa subunit, Activator 1 40 kDa subunit, Activator 1 subunit 2

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  WB analysis using 84892-1-RR (same clone as 84892-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84892-1-RR (RFC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01625-2

  Sandwich ELISA standard curve of MP01625-2, Human RFC2 Recombinant Matched Antibody Pair - PBS only. 84892-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0663. 84892-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP01625-2

  The mean RFC2 concentration was determined to be 55.11 ng/mL in HepG2 cell extract based on a 1.00 mg/mL extract load, 130.72 ng/mL in K-562 cell extract based on a 2.40 mg/mL extract load and 43.49 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

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  IHC staining of mouse ovary using 84892-1-RR (same clone as 84892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84892-1-RR (RFC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 84892-1-RR (same clone as 84892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84892-1-RR (RFC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84892-1-RR (same clone as 84892-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RFC2 antibody (84892-1-RR, Clone: 242503E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84892-1-RR (same clone as 84892-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC2 antibody (84892-1-RR, Clone: 242503E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84892-1-RR (same clone as 84892-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RFC2 Recombinant antibody (84892-1-RR, Clone:242503E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84892-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01625-2

  Cytometric bead array standard curve of MP01625-2, RFC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84892-1-PBS. Detection antibody: 84892-2-PBS. Standard: Ag0663. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84892-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84892-1-PBS against Human RFC2 was performed. The affinity constant is 1.24 nM.

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  Affinity and Kinetic Characterization of 84892-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84892-1-RR against Human RFC2 were performed. The affinity constant is 0.775 nM.

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Geprüfte Anwendungen

Produktinformation

84892-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test RFC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RFC2 fusion protein Ag0663
• Vollständiger Name: replication factor C (activator 1) 2, 40kDa
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC002813
• Gene symbol: RFC2
• Gene ID (NCBI): 5982
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RFC2, also named RFC40, belongs to the activator 1 small subunits family. It is a 39 kDa Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).